Journal: Human reproduction (Oxford, England)

Article Title: Distribution of decidual natural killer cells and macrophages in the neighbourhood of the trophoblast invasion front: a quantitative evaluation.

doi: 10.1093/humrep/det353

Figure Lengend Snippet: Figure 5 Distribution of dMph. (a) Cryostat section from a cell column at a first trimester placentation site double-stained with an anti-CD163 antibody (red) and an anti-HLA-G antibody (dark blue) as a sample image. Scale bar ¼ 100 mm. (b) Box plots of normalized areal cell densities s of dMph at first trimester placentation sites according to Equation (15), in five trophoblast neighbourhood rings according to Equation (17), are depicted. The dashed line s ¼ 1 represents the average areal cell density. #P ¼ 0.05, ##P ¼ 0.01 compared with 1 by Student’s t-tests (H0:m ¼ 1). ANOVA analysis showed a high significance,P ≤0.01.*P ¼ 0.05,**P ¼ 0.01ofpair-wiseTukeyhonestsignificantdifferenceposthoctests(s ¼ 1).(c)DistributionofdMphinco-culturesof first trimester decidua parietalis with first trimester villous explants using cryostat sections from co-cultures double-stained with an anti-CD163 antibody and an anti-HLA-G antibody. Box plots of normalized areal cell densities s. #P ¼ 0.05, ##P ¼ 0.01 compared with 1 by Student’s t-tests (H0:m ¼ 1). ANOVA analysis showed a high significance, P ≤0.01. **P ¼ 0.01 of pair-wise Tukey honest significant difference post hoc tests (s ¼ 1). Open circle is an outlier. (d) Distribution of dMph in first trimester decidua parietalis determined by using cryostat sections from tissue fragments stained with an anti-CD163 antibody. Box plots of normalized areal cell densities s. P . 0.05 by ANOVA test. Filled circle is an extreme value.

Article Snippet: The following antibodies were used: † monoclonal mouse anti-human CD56 (clone 123C3; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), dilution: 1:400, final concentration: 0.5 mg/ml; † monoclonal mouse anti-human CD163 (clone 5C6-FAT; Acris Antibodies GmbH, Hiddenhausen, Germany), dilution: 1:500, final concentration: 0.4 mg/ml; † monoclonal mouse anti-human HLA-G (clone 4H84; Exbio, Praha, Czech Republic; supplied by Eubio, Vienna, Austria), dilution: 1:500, final concentration: 2 mg/ml.

Techniques: Staining

Journal: OncoImmunology

Article Title: IL-32 induces indoleamine 2,3-dioxygenase+CD1c+ dendritic cells and indoleamine 2,3-dioxygenase+CD163+ macrophages: Relevance to mycosis fungoides progression

doi: 10.1080/2162402x.2016.1181237

Figure Lengend Snippet: Figure 2. Changes of CD14, CD163, and CD68 expression on monocyte-derived cells

Article Snippet: Antibodies used were: CD11c (B-ly6, BD Pharmingen) 1:100, CD1c (AD58E7, Miltenyi Biotec) 1:10, IDO (10.1, LSBio) 1:100, or CD163 (5C6-FAT, Acris Antibodies) 1:100.

Techniques: Expressing, Derivative Assay

Journal: Journal of Neuropathology and Experimental Neurology

Article Title: Serum Amyloid A Is Present in Human Saccular Intracranial Aneurysm Walls and Associates With Aneurysm Rupture

doi: 10.1093/jnen/nlab086

Figure Lengend Snippet: Primary Antibodies Used in the Immunostainings

Article Snippet: CD163 , 5C6-FAT , 20 , Novus Biologicals (Littleton, CO).

Techniques: Concentration Assay

Journal: Journal of Neuropathology and Experimental Neurology

Article Title: Serum Amyloid A Is Present in Human Saccular Intracranial Aneurysm Walls and Associates With Aneurysm Rupture

doi: 10.1093/jnen/nlab086

Figure Lengend Snippet: Correlations of positively stained area of apolipoprotein A-1 (A) , apolipoprotein B-100 (apoB-100) (B) , oxidized low-density lipoprotein (C) , and adipophilin (D) and number of CD3-positive (E) , CD68-positive (F) , and CD163-positive (G) cells with serum amyloid A scores in 36 saccular intracranial aneurysm walls. Spearman’s correlation test. *p = 0.006, **p = 0.001, ***p < 0.001, †R = 0.452, ††R = 0.526, †††R = 0.635, ††††R = 0.585, ‡R = 0.624, ‡‡R = 0.732, ‡‡‡R = 0.756.

Article Snippet: CD163 , 5C6-FAT , 20 , Novus Biologicals (Littleton, CO).

Techniques: Staining